Abstract: The Hongtoushan Cu-Zn deposit, located in the Hongtoushan rock formation of Archean greenstone in North Liaoning Province, is a large-scale ore deposit with great industrial significance. According to the analysis of H, O, S and Pb isotope characteristics, accompanying with the macroscopic natures, such as the orebody shape, ore texture, structure and wall-rock alteration, it is considered that the matter source of the Hongtoushan Cu-Zn deposit is the Neoarchean volcanic-sedimentary rock series. The ore-forming fluid is originated from atmospheric water and metamorphic water. The heat for ore-forming is from metamorphism. Thus the Hongtoushan Cu-Zn deposit belongs to stratabound type with volcanic-sedimentary and metamorphic hydrothermal overprinting.