Abstract: With a review on the current situation of testing techniques and application for fluid inclusion from petroliferous basins in oil and gas accumulation study, combined with the research experience in Tarim Basin, Junggar Basin and East China Sea Shelf Basin, this paper discusses the measurability of inclusions in quartz secondary enlargement edge, the selection of homogenization temperature test objects, the influence of later evolution on inclusion color analysis, as well as the error of visual identification of inclusion fluorescence colors. Since the GOI value of inclusions is affected by multiple factors such as in-situ stress field and heterogeneity of reservoir, the method should be carefully used in the study of filling strength or paleo-reservoir. It is suggested that the utilization of inclusion homogenization temperature data in discontinuous buried reservoirs be fully combined with the evolution of reservoir forming conditions such as trap evolution, transport system evolution and hydrocarbon generation and expulsion evolution of source rocks. It is not propose to simply use homogenization temperature to determine the reservoir forming stages.