Abstract: The Qingchengzi orefield in eastern Liaoning Province is an important gold polymetallic orefield with complex mineralization. The Baiyun and Xiaotongjiapuzi gold deposits are chosen to study the S-D-O isotopes. The results show that the S isotope content in Xiaotongjiapuzi gold deposit is 1.87‰ - 16‰, without distinct tower distribution, while that in Baiyun gold deposit ranges from -10.3‰ to +1.9‰. The D-O isotope test results indicate that the δD content is from -108.3‰ to -74‰, and the δ¹⁸O content is 8‰ - 15.9‰ in the quartz of Baiyun gold deposit. The D-O isotopes of quartz in Xiaotongjiapuzi gold deposit is dispersely distributed. According to the comprehensive analysis, it is believed that Xiaotongjiapuzi gold deposit represents the early-stage mineralization, related to metamorphism, and Baiyun gold deposit represents the late-stage mineralization. The mineralization of stratiform Pb-Zn deposit and Xiaotongjiabaozi gold deposit in the orefield might occur in the same period.