Abstract: The Henanzhuang Gold Deposit is located on the Qinling tectonic belt in southernmost North China Plate with the host rock of a set of calc-muddy carbonate rock formation. The mineralization is controlled by strata and structure. The sources and metallogenic conditions of ore-forming fluid are studied by analyzing the trace, rare earth elements and C-H-O-S isotopes. The results show that the ore-forming fluid derived from magma is affected by later metamorphism and sedimentary reformation. The C and partial O elements come from the dissolution of marine carbonate, and catalyze the enrichment of Au. The S isotope and trace elements data indicate the deposit was formed in the anoxic reducing environment with the ore-forming fluid derived from deep crust or mantle. The geological and geochemical characteristics of the Henanzhuang Gold Deposit are similar to those of altered rock-type gold deposits, thus it should be classified as the latter.